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Material and Methods Reagents and Antibodies Dulbecco's Modified Eagle Medium (DMEM), DMEM/Nutrient Mixture F-12 (DMEM/F-12), Essential 8 and Neurobasal-A medium, Essentiel 8 Supplement, B-27 Supplement, fetal calf serum (FCS), trypsin, Glutamax, penicillin, streptomycin were from Gibco

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